Graphene-oxide Assisted SELEX (GO-SELEX) for Screening of Aptamers and Its Application on Biosensors:
Man Bock Gu is a Professor and the Director of BK21PLUS for Biotechnology at Korea University, Seoul, Rep. of Korea. He received his Ph.D. in the Department of Chemical Engineering in the University of Colorado at Boulder in 1994, and completed his postdoctoral research in the Department of Chemical Engineering in the University of Delaware in 1995. He had been a PI of the National Research Lab (NRL) on Biosensors, while he was a professor at Gwangju Institute of Science and Technology (GIST), Gwangju, Rep. of Korea, from 1996 to 2005. He was a recipient of Alexander von Humboldt research fellowship in Berlin, Germany in 2001-2002. Prof. Gu was a president of Korean Biochip Society in 2014 and is a regular member of both the Korean Academy of Science and Technology and the National Academy of Engineering of Korea since 2015. He is an executive editor (Co-Editors-in chief) of the journal Biosensors and Bioelectronics. He has been an organizing committee member of World Congress on Biosensors since 2012, and is a Congress chair of Biosensors 2020, as an Asian Congress Chair, which will be held in Busan, Rep. of Korea, on May 26-28, 2020.
Google scholar: https://scholar.google.com/citations?user=prbLT_cAAAAJ&hl=ko&oi=ao
Aptamers, small sizes of ssDNAs or RNAs, have been well known as bioreceptor molecules with high affinity and specificity over various targets since 1990. One of the tedious and sometimes serious hurdles to develop aptamers is to immobilize the target moieties to the solid matrixes, mainly for the separation of unbound library from bound ones. There have been a lot of efforts to elaborate many special methods to develop aptamers without target immobilization. We have also successfully developed an immobilization-free screening of aptamers by using graphene-oxide (GO-SELEX) for the first time in world and this method has been widely and successfully used not only in my lab but also world-widely. Interestingly, we have found out that this method is specifically useful for finding a pair of aptamers binding at different binding sites of the same target moiety simultaneously, which will lead to the sandwich-type binding in a form of aptamer1-target-aptamer2. In this talk, it will be presented how we started this GO-SELEX method and the successful outcomes of sandwich-type binding and their application to electrochemical and lateral flow biosensor platforms for a few different targets, including disease biomarker proteins and pandemic avian influenza viruses, in addition to show some examples of a few different organic/inorganic, nano/micro-structured surfaces of the electrodes with a new mini-potentiostat.